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The data show that all the Neanderthal remains are from a much earlier period ( Previous dating of the Vi-207 and Vi-208 Neanderthal remains from Vindija Cave (Croatia) led to the suggestion that Neanderthals survived there as recently as 28,000–29,000 B. Subsequent dating yielded older dates, interpreted as ages of at least ∼32,500 B. We have redated these same specimens using an approach based on the extraction of the amino acid hydroxyproline, using preparative high-performance liquid chromatography (Prep-HPLC).
This method is more efficient in eliminating modern contamination in the bone collagen. P., suggesting the Vindija Neanderthals did not live more recently than others across Europe, and probably predate the arrival of anatomically modern humans in Eastern Europe.
All dates obtained on the four Neanderthal specimens at the ORAU are reported in Table 2.
We also list the dates obtained on two other hominin samples: Vi-75-G3/h-203, analyzed at the Uppsala Radiocarbon Laboratory (Sweden) (23), and Vi-2291-18 (level G, sublayer unknown), prepared at the Max Planck Institute, Leipzig, and dated at the ORAU (24).
The mitochondrial DNA sequence of Vi-207 was identical to Vi-33.25 and Feldhofer 1 mitochondrial genomes, whereas Vi-*28 had an identical mitochondrial sequence to Vi-33.17 (, Fig. Both Vi-33.25 and Vi-33.17 were found in layer I of Vindija Cave.
As previously published, Vi-33.19 has the same mitochondrial sequence as Vi-33.16 (22).
Two specimens, Vi-207 and Vi-208, were originally directly AMS dated in the late 1990s at the Oxford Radiocarbon Accelerator Unit (ORAU). If the dates are even approximately correct, however, it makes them the most recent known Neanderthals.
Only 101 samples produced identifiable spectra; a summary of all taxa identified by Zoo MS is shown in ), which again highlights the use of applying such techniques to groups of unidentified Paleolithic bone samples.We applied zooarchaeology by mass spectrometry (Zoo MS) to find additional hominin remains.We identified one bone that is Neanderthal, based on its mitochondrial DNA, and dated it directly to 46,200 ± 1,500 B. We also attempted to date six early Upper Paleolithic bone points from stratigraphic units G. in Europe witnessed the so-called biocultural transition from the Middle to early Upper Paleolithic, when incoming anatomically modern humans displaced Neanderthal groups across the continent (1, 2).The different sample pretreatments are also indicated in Table 2.Vi-208 and Vi-207 produced hydroxyproline dates of 42,700 ± 1,600 and 43,900 ± 2,000 B. These ages are significantly older than any of the dates obtained previously for these specimens using the AG (gelatinized filtered collagen) and AF (ultrafiltered collagen) procedures, and this strongly suggests that noncollagenous high molecular weight contaminants, probably crosslinked to the collagen, were still present in the sample previously dated.